Isolation and Purification of Antibiotic Material from Physarum gyrosum
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چکیده
منابع مشابه
Isolation and purification of antibiotic material from Physarum gyrosum.
The myxomycete Physarum gyrosum was cultured in its plasmodial stage on agar plates containing 0.025 M phosphate buffer at pH 6.5, 2% bakers' yeast, and 0.2% glucose and was supplemented with live Escherichia coli. Extracts of these plasmodia contained several antibiotic substances. Antibiotic materials were partially purified by dialysis of the agar medium-mold mixture, evaporation of the dial...
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The physical and nutritional requirements of the antibiotic-producing slime mold Physarum gyrosum were examined to develop a liquid medium for this myxomycete. Liquid culture is desired to expedite a useful scale of production of antibiotic materials for ease of isolation and structure study. Culture conditions were selected to favor antibiotic production rather than maximum growth. The medium ...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1973
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.4.2.160